Chemical Dosimetry Using Bisbenzimidazoles: Solvent-Dependent Fluorescence Response of Hoechst 33258 to Radiation Exposure

Bisbenzimidazoles have a broad spectrum of potential applications: radioprotectors, drug delivery vectors, antiviral agents, etc. At the same time, they seem to be promising fluorescent probes for radiation measurements. Therefore, in present work, response X-ray irradiation Hoechst 33258, one most widely known representatives bisbenzimidazole family, was studied first time. Irradiation dye performed aqueous and organic solutions (DMSO glycerol), as well their mixtures. It is shown that reaction exposure very versatile may controlled by solvent properties, which makes it possible build relationships between absorbed dose wide variety parameters its fluorescence signal. For example, induce quenching caused degradation dye, change position band maximum due modification molecules or radiation-induced changes properties medium, flare-up mediated pH.